The Effect of Reperfusion after Short Time Ischemic on Spinal Cord on Rat

Introduction: Ischemia plays an important role in the development of pathologic in various neuropathies, and reperfusion improves the pathological and physiological abnormalities of ischemic nerves. In this study, the effect of shortreperfusion time after short time ischemic on spinal cord of rat was investigated. Methods: Eighteen rats (Sprague-Dawley, 250-350 g), were divided into 3 groups: group I (surgical sham), group II (60 minutes of ischemic abdominal aorta, followed by 48 hours reperfusion), group III (60 minutes of ischemia of the abdominal aorta, followed by 72 hours of reperfusion). All animals were evaluated by (Motor Deficit Index or MDI), Malondialdehide (MDA), Total antioxidant capacity (TAC), and histopathological parameters within 48 hours or 72 hours after surgery. Results: The mean of MDI scores in groups II and III was not statistically significant (P > 0.05). The mean number of normal motor neueons of spinal cord in group III was significantly lower than group II (P < 0.05). The mean of MDAplasma level in group III was higher than group II, and this difference was significant (P < 0.05). The mean TAC plasma level in group III was significantly lower than group II (P < 0.05). Conclusions: These results indicate that increasing the time of reperfusion from 48 to 72 hours may increase paralysis in the hind limb of the rat